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pcmv ha mammalian expression vector  (TaKaRa)


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    Structured Review

    TaKaRa pcmv ha mammalian expression vector
    Pcmv Ha Mammalian Expression Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 770 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcmv+ha+mammalian+expression+vector/pCMV-HA+Vector+Set/bio_rxiv__2025__08__02__668297-212-1-15
    Average 96 stars, based on 770 article reviews
    pcmv ha mammalian expression vector - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Expressing:

    Article Title: Pathogenic DVL frameshifting variants in Robinow syndrome disrupt WNT signaling and cellular dynamics
    Article Snippet: .. The pCMV-HA mammalian expression vector containing an N-terminal hemagglutinin (HA) epitope tag was purchased from Clontech Inc. (Mountain View, CA, Cat. No. 631604). ..

    Article Title: FKBP-L polypeptides and uses in angiogenesis-mediated disorders
    Article Snippet: .. In order to generate the HA-tagged FKBPL plasmid, the FKBPL cDNA (polynucleotide SEQ ID NO:31; polypeptide variant variant Thr182, Gly186; SEQ ID NO:2) was excised from pUC18 by digestion with BamH1, blunt ended and directionally cloned into a blunt ended Sall site of pCMV-HA mammalian expression vector (Clontech, U.K.). ..

    Article Title: HTLV-1 subgroups associated with the risk of HAM/TSP are related to viral and host gene expression in peripheral blood mononuclear cells, independent of the transactivation functions of the viral factors.
    Article Snippet: Among human Tcell leukemia virus type 1 (HTLV1)-infected individuals, the risk of developing HTLV-1associated myelopathy/tropical spastic paraparesis (HAM/ TSP) across lifetime differs between ethnic groups.. There is an association between HTLV-1 tax gene subgroups (subgroup-A or subgroup-B) and the risk of HAM/TSP in the Japanese population.. In this study, we investigated the fulllength proviral genome sequences of various HTLV-1infected cell lines and patient samples.

    Article Title: Nuclear Thioredoxin‐1 Overexpression Attenuates Alcohol‐Mediated Nrf2 Signaling and Lung Fibrosis
    Article Snippet: .. Trx1 cDNA was inserted between the cytomegalovirus (CMV) promoter and the simian virus (SV‐40) terminator of a pCMV‐Myc or a pCMV‐HA mammalian expression vector purchased from Clontech Laboratories (Mountain View, CA; Go et al., ). ..

    Article Title: LncRNAs-directed PTEN enzymatic switch governs epithelial–mesenchymal transition
    Article Snippet: Plasmids construction, transfection and lentiviral transduction The full-length of MEX3C and PTEN mammalian expression vectors were obtained from OriGene. .. Ubiquitin and its corresponding mutants were constructed into pCMV-HA mammalian expression vector (Clontech). ..

    Article Title: FKBP-L and uses thereof
    Article Snippet: .. In order to generate the HA-tagged FKBPL plasmid, the FKBPL cDNA (polynucleotide SEQ ID NO:31; polypeptide variant Thr182, Gly186; SEQ ID NO:2) was excised from pUC18 by digestion with BamHI, blunt ended and directionally cloned into a blunt ended SalI site of pCMV-HA mammalian expression vector (Clontech, U.K.). ..

    Plasmid Preparation:

    Article Title: Pathogenic DVL frameshifting variants in Robinow syndrome disrupt WNT signaling and cellular dynamics
    Article Snippet: .. The pCMV-HA mammalian expression vector containing an N-terminal hemagglutinin (HA) epitope tag was purchased from Clontech Inc. (Mountain View, CA, Cat. No. 631604). ..

    Article Title: FKBP-L polypeptides and uses in angiogenesis-mediated disorders
    Article Snippet: .. In order to generate the HA-tagged FKBPL plasmid, the FKBPL cDNA (polynucleotide SEQ ID NO:31; polypeptide variant variant Thr182, Gly186; SEQ ID NO:2) was excised from pUC18 by digestion with BamH1, blunt ended and directionally cloned into a blunt ended Sall site of pCMV-HA mammalian expression vector (Clontech, U.K.). ..

    Article Title: HTLV-1 subgroups associated with the risk of HAM/TSP are related to viral and host gene expression in peripheral blood mononuclear cells, independent of the transactivation functions of the viral factors.
    Article Snippet: Among human Tcell leukemia virus type 1 (HTLV1)-infected individuals, the risk of developing HTLV-1associated myelopathy/tropical spastic paraparesis (HAM/ TSP) across lifetime differs between ethnic groups.. There is an association between HTLV-1 tax gene subgroups (subgroup-A or subgroup-B) and the risk of HAM/TSP in the Japanese population.. In this study, we investigated the fulllength proviral genome sequences of various HTLV-1infected cell lines and patient samples.

    Article Title: Nuclear Thioredoxin‐1 Overexpression Attenuates Alcohol‐Mediated Nrf2 Signaling and Lung Fibrosis
    Article Snippet: .. Trx1 cDNA was inserted between the cytomegalovirus (CMV) promoter and the simian virus (SV‐40) terminator of a pCMV‐Myc or a pCMV‐HA mammalian expression vector purchased from Clontech Laboratories (Mountain View, CA; Go et al., ). ..

    Article Title: LncRNAs-directed PTEN enzymatic switch governs epithelial–mesenchymal transition
    Article Snippet: Plasmids construction, transfection and lentiviral transduction The full-length of MEX3C and PTEN mammalian expression vectors were obtained from OriGene. .. Ubiquitin and its corresponding mutants were constructed into pCMV-HA mammalian expression vector (Clontech). ..

    Article Title: FKBP-L and uses thereof
    Article Snippet: .. In order to generate the HA-tagged FKBPL plasmid, the FKBPL cDNA (polynucleotide SEQ ID NO:31; polypeptide variant Thr182, Gly186; SEQ ID NO:2) was excised from pUC18 by digestion with BamHI, blunt ended and directionally cloned into a blunt ended SalI site of pCMV-HA mammalian expression vector (Clontech, U.K.). ..

    Variant Assay:

    Article Title: FKBP-L polypeptides and uses in angiogenesis-mediated disorders
    Article Snippet: .. In order to generate the HA-tagged FKBPL plasmid, the FKBPL cDNA (polynucleotide SEQ ID NO:31; polypeptide variant variant Thr182, Gly186; SEQ ID NO:2) was excised from pUC18 by digestion with BamH1, blunt ended and directionally cloned into a blunt ended Sall site of pCMV-HA mammalian expression vector (Clontech, U.K.). ..

    Article Title: FKBP-L and uses thereof
    Article Snippet: .. In order to generate the HA-tagged FKBPL plasmid, the FKBPL cDNA (polynucleotide SEQ ID NO:31; polypeptide variant Thr182, Gly186; SEQ ID NO:2) was excised from pUC18 by digestion with BamHI, blunt ended and directionally cloned into a blunt ended SalI site of pCMV-HA mammalian expression vector (Clontech, U.K.). ..

    Clone Assay:

    Article Title: FKBP-L polypeptides and uses in angiogenesis-mediated disorders
    Article Snippet: .. In order to generate the HA-tagged FKBPL plasmid, the FKBPL cDNA (polynucleotide SEQ ID NO:31; polypeptide variant variant Thr182, Gly186; SEQ ID NO:2) was excised from pUC18 by digestion with BamH1, blunt ended and directionally cloned into a blunt ended Sall site of pCMV-HA mammalian expression vector (Clontech, U.K.). ..

    Article Title: HTLV-1 subgroups associated with the risk of HAM/TSP are related to viral and host gene expression in peripheral blood mononuclear cells, independent of the transactivation functions of the viral factors.
    Article Snippet: Among human Tcell leukemia virus type 1 (HTLV1)-infected individuals, the risk of developing HTLV-1associated myelopathy/tropical spastic paraparesis (HAM/ TSP) across lifetime differs between ethnic groups.. There is an association between HTLV-1 tax gene subgroups (subgroup-A or subgroup-B) and the risk of HAM/TSP in the Japanese population.. In this study, we investigated the fulllength proviral genome sequences of various HTLV-1infected cell lines and patient samples.

    Article Title: FKBP-L and uses thereof
    Article Snippet: .. In order to generate the HA-tagged FKBPL plasmid, the FKBPL cDNA (polynucleotide SEQ ID NO:31; polypeptide variant Thr182, Gly186; SEQ ID NO:2) was excised from pUC18 by digestion with BamHI, blunt ended and directionally cloned into a blunt ended SalI site of pCMV-HA mammalian expression vector (Clontech, U.K.). ..

    Amplification:

    Article Title: HTLV-1 subgroups associated with the risk of HAM/TSP are related to viral and host gene expression in peripheral blood mononuclear cells, independent of the transactivation functions of the viral factors.
    Article Snippet: Among human Tcell leukemia virus type 1 (HTLV1)-infected individuals, the risk of developing HTLV-1associated myelopathy/tropical spastic paraparesis (HAM/ TSP) across lifetime differs between ethnic groups.. There is an association between HTLV-1 tax gene subgroups (subgroup-A or subgroup-B) and the risk of HAM/TSP in the Japanese population.. In this study, we investigated the fulllength proviral genome sequences of various HTLV-1infected cell lines and patient samples.

    Virus:

    Article Title: Nuclear Thioredoxin‐1 Overexpression Attenuates Alcohol‐Mediated Nrf2 Signaling and Lung Fibrosis
    Article Snippet: .. Trx1 cDNA was inserted between the cytomegalovirus (CMV) promoter and the simian virus (SV‐40) terminator of a pCMV‐Myc or a pCMV‐HA mammalian expression vector purchased from Clontech Laboratories (Mountain View, CA; Go et al., ). ..

    Ubiquitin Proteomics:

    Article Title: LncRNAs-directed PTEN enzymatic switch governs epithelial–mesenchymal transition
    Article Snippet: Plasmids construction, transfection and lentiviral transduction The full-length of MEX3C and PTEN mammalian expression vectors were obtained from OriGene. .. Ubiquitin and its corresponding mutants were constructed into pCMV-HA mammalian expression vector (Clontech). ..

    Construct:

    Article Title: LncRNAs-directed PTEN enzymatic switch governs epithelial–mesenchymal transition
    Article Snippet: Plasmids construction, transfection and lentiviral transduction The full-length of MEX3C and PTEN mammalian expression vectors were obtained from OriGene. .. Ubiquitin and its corresponding mutants were constructed into pCMV-HA mammalian expression vector (Clontech). ..

    other:

    Article Title: LncRNAs-directed PTEN enzymatic switch governs epithelial-mesenchymal transition.
    Article Snippet: 3 independent biological repeats for each experimental condition were shown. b In vitro dephosphorylation assay were performed with 32P-labled substrates in the presence of unmodified or polyubiquitinated PTEN, and analyzed by IB or autoradiography. c Stability of TWIST1 and SNAI1 proteins after CHX treatment (100 μg/mL) for indicated time in glucose-starved MCF10A PTEN+/+ or MCF10A PTEN-/-cells followed by glucose re-stimulation (5mM or 25mM). d, e IB detection of indicated proteins in MCF10A PTEN+/+ or MCF10A PTEN-/-cells transfected with indicated vectors followed by glucose starvation with or without MG132 pre-treatment (5 μM, 6 h) and re-stimulation of glucose (5, 25mM, 1 h). f IB detection using indicated antibodies in MCF10A PTEN+/+ and MCF10A PTEN-/- cells transfected with indicated expression vectors and siRNAs subjected to glucose starvation and followed by glucose re-stimulation (5 or 25mM) Cell Research (2019) 29:286 – 304 mutants were constructed into pCMV-HA mammalian expression vector (Clontech).



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    TaKaRa mammalian expression vector pcmv myc
    Confocal microscopy of HeLa cells transfected with ( A ) <t>pCMV-HA-FOX-2</t> V1 and pcDNA1-FLAG-SCA1-Q30 or pcDNA1-FLAG-SCA1-Q82, or ( B ) <t>pCMV-MYC-FOX-2</t> cyt and pcDNA1-FLAG-SCA1-Q30 or pcDNA1-FLAG-SCA1-Q82, and ( C ) pCMV-MYC-TIAR and pcDNA1-FLAG-SCA1-Q30 or pcDNA1-FLAG-SCA1-Q82, respectively. Forty-eight hours post transfection cells were fixed and prepared for microscopic analyses. Proteins were visualized using the respective antibodies against the tag as described in . Nuclei were stained using Hoechst. Bars represent 20 µm.
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    Becton Dickinson pcmv-ha mammalian expression vector
    A: Yeast two hybrid assay. CDNA for CypB was cloned in frame into the yeast expression vector (pGBKT7) that harbours the GAL4 activation domain. The recombinant plasmids plus Human Kidney Matchmaker cDNA Library were co-transformed into AH109 yeast strain. The co-transformed cells were selected on Ade-/Leu-/His-/Trp-, 10 µM Aminotriazol dropout plates to monitor for growth. The positive control represents co-transformation of p53 and T-antigen in two-hybrid expression vectors, pGBKT7-P53 and pGADT7 (BD <t>Biosciences,</t> <t>Clontech),</t> respectively. B: GST-pull-down assays. CypB was fused in frame with the GST gene. GST and GST-HCypB products were immobilized on Sepharose 4B and incubated with COS-7 cells lysates expressing Na/K-β1 <t>(pCMV-HA-Na/K).</t> The bound proteins were analyzed by immunoblotting with anti-Na/K-β1 rabbit polyclonal antibody. C–E: Co-Immunoprecipitation assays. HK-2 lysates were immunoprecipitated with mouse monoclonal antibodies against Na/K-β1 or Na/K-α1 and rabbit polyclonal antibody against CypB. The immunoprecipitates were subjected to Western blotting analyses, as indicated in the figure. As control, ChromePure mouse IgG and ChromePure rabbit IgG were used. Figures 1B to 1E are representative of at least three independent experiments.
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    Image Search Results


    Confocal microscopy of HeLa cells transfected with ( A ) pCMV-HA-FOX-2 V1 and pcDNA1-FLAG-SCA1-Q30 or pcDNA1-FLAG-SCA1-Q82, or ( B ) pCMV-MYC-FOX-2 cyt and pcDNA1-FLAG-SCA1-Q30 or pcDNA1-FLAG-SCA1-Q82, and ( C ) pCMV-MYC-TIAR and pcDNA1-FLAG-SCA1-Q30 or pcDNA1-FLAG-SCA1-Q82, respectively. Forty-eight hours post transfection cells were fixed and prepared for microscopic analyses. Proteins were visualized using the respective antibodies against the tag as described in . Nuclei were stained using Hoechst. Bars represent 20 µm.

    Journal: PLoS ONE

    Article Title: FOX-2 Dependent Splicing of Ataxin-2 Transcript Is Affected by Ataxin-1 Overexpression

    doi: 10.1371/journal.pone.0037985

    Figure Lengend Snippet: Confocal microscopy of HeLa cells transfected with ( A ) pCMV-HA-FOX-2 V1 and pcDNA1-FLAG-SCA1-Q30 or pcDNA1-FLAG-SCA1-Q82, or ( B ) pCMV-MYC-FOX-2 cyt and pcDNA1-FLAG-SCA1-Q30 or pcDNA1-FLAG-SCA1-Q82, and ( C ) pCMV-MYC-TIAR and pcDNA1-FLAG-SCA1-Q30 or pcDNA1-FLAG-SCA1-Q82, respectively. Forty-eight hours post transfection cells were fixed and prepared for microscopic analyses. Proteins were visualized using the respective antibodies against the tag as described in . Nuclei were stained using Hoechst. Bars represent 20 µm.

    Article Snippet: Subsequently, the relevant DNA fragment was purified and subcloned into the Sal I/ Not I sites of the mammalian expression vector pCMV-MYC (Clontech; Mountain View, USA).

    Techniques: Confocal Microscopy, Transfection, Staining

    ( A ) Schematic illustration of ATXN1 interactions. Black lines represent interactions reported by Lim and co-workers , the blue line represents the investigated interaction in this study. ( B ) Schematic view of ATXN2 regions used in Y2H studies as described earlier , , . ( C; D ) L40ccua yeast cells expressing the corresponding LexA-ATXN2 and AD-FOX-2 fusion proteins were spotted onto selective media or membrane as indicated and the activity of the reporter genes was monitored. ( E ) HeLa cell lysates were incubated with an antibody directed against FOX-2 (Bethyl) and membranes were treated with an anti-ATXN2 antibody (BD-Biosciences) to detect precipitated protein. ( F ) HeLa cells were transfected with pCMV-MYC-ATXN2-Q22 or co-transfected with pCMV-MYC-ATXN2-Q22 and pcDNA1-FLAG-SCA1-Q30 or pcDNA1-FLAG-SCA1-Q82, respectively. Forty-eight hours post transfection cells were fixed and prepared for microscopic analyses. Nuclei were stained using Hoechst. Bars represent 20 µm.

    Journal: PLoS ONE

    Article Title: FOX-2 Dependent Splicing of Ataxin-2 Transcript Is Affected by Ataxin-1 Overexpression

    doi: 10.1371/journal.pone.0037985

    Figure Lengend Snippet: ( A ) Schematic illustration of ATXN1 interactions. Black lines represent interactions reported by Lim and co-workers , the blue line represents the investigated interaction in this study. ( B ) Schematic view of ATXN2 regions used in Y2H studies as described earlier , , . ( C; D ) L40ccua yeast cells expressing the corresponding LexA-ATXN2 and AD-FOX-2 fusion proteins were spotted onto selective media or membrane as indicated and the activity of the reporter genes was monitored. ( E ) HeLa cell lysates were incubated with an antibody directed against FOX-2 (Bethyl) and membranes were treated with an anti-ATXN2 antibody (BD-Biosciences) to detect precipitated protein. ( F ) HeLa cells were transfected with pCMV-MYC-ATXN2-Q22 or co-transfected with pCMV-MYC-ATXN2-Q22 and pcDNA1-FLAG-SCA1-Q30 or pcDNA1-FLAG-SCA1-Q82, respectively. Forty-eight hours post transfection cells were fixed and prepared for microscopic analyses. Nuclei were stained using Hoechst. Bars represent 20 µm.

    Article Snippet: Subsequently, the relevant DNA fragment was purified and subcloned into the Sal I/ Not I sites of the mammalian expression vector pCMV-MYC (Clontech; Mountain View, USA).

    Techniques: Expressing, Activity Assay, Incubation, Transfection, Staining

    A: Yeast two hybrid assay. CDNA for CypB was cloned in frame into the yeast expression vector (pGBKT7) that harbours the GAL4 activation domain. The recombinant plasmids plus Human Kidney Matchmaker cDNA Library were co-transformed into AH109 yeast strain. The co-transformed cells were selected on Ade-/Leu-/His-/Trp-, 10 µM Aminotriazol dropout plates to monitor for growth. The positive control represents co-transformation of p53 and T-antigen in two-hybrid expression vectors, pGBKT7-P53 and pGADT7 (BD Biosciences, Clontech), respectively. B: GST-pull-down assays. CypB was fused in frame with the GST gene. GST and GST-HCypB products were immobilized on Sepharose 4B and incubated with COS-7 cells lysates expressing Na/K-β1 (pCMV-HA-Na/K). The bound proteins were analyzed by immunoblotting with anti-Na/K-β1 rabbit polyclonal antibody. C–E: Co-Immunoprecipitation assays. HK-2 lysates were immunoprecipitated with mouse monoclonal antibodies against Na/K-β1 or Na/K-α1 and rabbit polyclonal antibody against CypB. The immunoprecipitates were subjected to Western blotting analyses, as indicated in the figure. As control, ChromePure mouse IgG and ChromePure rabbit IgG were used. Figures 1B to 1E are representative of at least three independent experiments.

    Journal: PLoS ONE

    Article Title: Cyclophilin B Interacts with Sodium-Potassium ATPase and Is Required for Pump Activity in Proximal Tubule Cells of the Kidney

    doi: 10.1371/journal.pone.0013930

    Figure Lengend Snippet: A: Yeast two hybrid assay. CDNA for CypB was cloned in frame into the yeast expression vector (pGBKT7) that harbours the GAL4 activation domain. The recombinant plasmids plus Human Kidney Matchmaker cDNA Library were co-transformed into AH109 yeast strain. The co-transformed cells were selected on Ade-/Leu-/His-/Trp-, 10 µM Aminotriazol dropout plates to monitor for growth. The positive control represents co-transformation of p53 and T-antigen in two-hybrid expression vectors, pGBKT7-P53 and pGADT7 (BD Biosciences, Clontech), respectively. B: GST-pull-down assays. CypB was fused in frame with the GST gene. GST and GST-HCypB products were immobilized on Sepharose 4B and incubated with COS-7 cells lysates expressing Na/K-β1 (pCMV-HA-Na/K). The bound proteins were analyzed by immunoblotting with anti-Na/K-β1 rabbit polyclonal antibody. C–E: Co-Immunoprecipitation assays. HK-2 lysates were immunoprecipitated with mouse monoclonal antibodies against Na/K-β1 or Na/K-α1 and rabbit polyclonal antibody against CypB. The immunoprecipitates were subjected to Western blotting analyses, as indicated in the figure. As control, ChromePure mouse IgG and ChromePure rabbit IgG were used. Figures 1B to 1E are representative of at least three independent experiments.

    Article Snippet: BD Biosciences, clontech, Palo Alto, CA), was subcloned into the BglII restriction endonuclease site of pCMV-HA mammalian expression vector (BD Biosciences, clontech, Palo Alto, CA) to get the pCMV- Na/K-β1 -HA construct.

    Techniques: Y2H Assay, Clone Assay, Expressing, Plasmid Preparation, Activation Assay, Recombinant, cDNA Library Assay, Transformation Assay, Positive Control, Incubation, Western Blot, Immunoprecipitation